Recycle Gel Permeation Chromatography: Application to the Separation of Some Diastereoisomers Through Molecular Interaction Effects

Abstract

This paper deals with the separation of small molecules of very close molar volumes by recycle gel permeation chromatography. After a preliminary part where experimental conditions required for such separations are given, we present our first results about separations of diastereoisomers of some acetal-alcohols which form hydrogen bonds with the chromatographic solvent. Separations are interpreted by means of molecular interactions which lead to different average hydrodynamic volumes for the two components of each diastereoisomer mixture. We show theoretically that these differences are dependent upon the proton acceptor character and the molar volume of the GPC solvent and optimal conditions versus these parameters are given.