Prostaglandin and histamine release from stimulated rat peritoneal mast cells

Abstract

The production of five prostanoids (PGD₂, PGE₂, PGF_2α, 6-oxo-PGF_1α, and TxB₂) was examined after mast cell activation. Prostaglandin D₂ (PGD₂) was the major prostanoid produced after stimulation of rat peritoneal mast cells with the calcium ionophore A 23187, compound 48/80 or anti-rat IgE. The amount of PGD₂ generated was not dependent on the quantity of histamine released. The time course of PGD₂ production paralleled the release of histamine following activation with A 23187 or anti-rat IgE but with compound 48/80 release of histamine reached a maximum within 15 sec, whereas production of PGD₂ was apparent only after 5 min and was still increasing at 30 min.