A New Preparative Method for Microspectrophotometric DNA-Measurement in Megakaryocytes, Revealing a Significant Group of Cells with a DNA Content of 64 N

Abstract
Microspectrophotometric investigations of Feulgen-stained rat bone marrow megakaryocytes, comparing the traditional smear method and a new 1 G sedimentation and 40 degrees C evaporation (S. E.) method, have shown that the latter reveals a significant group of megakaryocytes (ca. 30 percent) with a DNA content of 64 N. This group is totally absent when the smear method is used.