Immunofluorescent localization of type IV collagen and laminin in human skin and its application in junctional zone pathology

Abstract

Purified antibodies against type IV collagen and laminin were used to localize basement membranes by indirect immunofluorescence in various anatomical regions of normal and diseased human skin. The 2 proteins showed extensive codistribution. A continuous linear staining was found along the epidermal-dermal junction and around hair follicles, sebaceous gland acini and small capillaries. The same proteins also surrounded individual cells such as those found in vessels, hair erector muscles and s.c. tissue. Blister formation in bullous pemphigoid left type IV collagen and laminin on the floor of the blister, while the bullous pemphigoid antigen as detected by human autoantibodies was found on both sides of the blister. In solid basal cell carcinoma a strong staining was found around all tumor islands as well as focally within the cell clusters. This suggests that the tumor cells produce these basement membrane proteins, but have lost, at least in part, control of polar deposition.