Kinetics of substrate utilization in adenine-limited chemostat cultures of Bacillus subtilis KYA741
- 1 April 1982
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 43 (4), 794-799
- https://doi.org/10.1128/aem.43.4.794-799.1982
Abstract
The specific rates of limiting substrate utilization were investigated in adenine- or glucose-limited chemostat cultures of Bacillus subtilis KYA741, an adenine-requiring strain, at 37 degrees C. With the glucose-limited cultures, the specific rate of glucose consumption versus dilution rate gave a linear relationship from which the true growth yield and maintenance coefficient were determined to be 0.09 mg of bacteria per mg of glucose and 0.2 mg of glucose per mg of bacteria per h, respectively. With the adenine-limited cultures, adenine as the limiting substrate was not completely consumed at lower dilution rates (e.g., D less than 0.1), unlike in the glucose-limited cultures. When a linear relationship of specific rate of adenine consumption versus dilution rate was extrapolated to zero dilution rate, a negative value for the specific rate of adenine consumption, -0.01 mg of adenine per mg of bacteria per h, was obtained, giving a true growth yield for adenine of 5.2 mg of bacteria per mg of adenine. On the other hand, the maintenance coefficient of oxygen uptake gave a positive value of 8.1 x 10(-3) mmol/mg of bacteria per h. Based on previous results showing that adenine is resupplied by lysing cells, we developed kinetic models of adenine utilization and cell growth that gave a good estimation of the peculiar behavior of cell growth and adenine utilization in adenine-limited chemostat cultures.This publication has 8 references indexed in Scilit:
- Substrate utilization kinetic model for biological treatment processBiotechnology & Bioengineering, 1980
- Growth kinetics under adenine-limiting conditions ofBacillus subtilis KYA 741, an adenine-requiring strainBiotechnology & Bioengineering, 1980
- Energetics of Microbacterium thermosphactum in glucose-limited continuous cultureApplied and Environmental Microbiology, 1979
- BIOENERGETIC ASPECTS OF AEROBIC GLUCOSE-METABOLISM OF ESCHERICHIA-COLI K-12 UNDER VARYING SPECIFIC GROWTH-RATES AND GLUCOSE CONCENTRATIONS1978
- Utilization of energy for growth and maintenance in continuous and batch cultures of microorganismsBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1973
- Reassessment of Maintenance and Energy Uncoupling in the Growth of Azotobacter vinelandiiJournal of General Microbiology, 1972
- ENERGETIC AND NUCLEIC ANALYSES OF A CHEMOSTATIC CULTURE OF AZOTOBACTER VINELANDIIThe Journal of General and Applied Microbiology, 1967
- The maintenance energy of bacteria in growing culturesProceedings of the Royal Society of London. B. Biological Sciences, 1965