A MODEL OF INSITU IMMUNE-COMPLEX GLOMERULONEPHRITIS IN THE RAT EMPLOYING CATIONIZED FERRITIN

Abstract

In the model described here the ability of cationized (basic) ferritin [F] to bind to the glomerular basement membrane and act as a planted antigen was exploited. This planted antigen was accessible to circulating antibody resulting in in situ immune complex [IC] formation. Perfusion of cationized F (isoelectric point > 9.5) directly into the renal arteries, followed by i.v. injection of anti-ferritin antibody resulted in induction of glomerulonephritis with heavy proteinuria lasting for about 3 wk. Fine granular deposition of antigen, antibody and rat complement component 3 along the glomerular capillary walls was seen by immunofluorescence. EM revealed the presence of subepithelial deposits containing F; the foot processes were fused. This demonstrates the potential role of basic antigens in in situ IC glomerulonephritis pathogenesis.