An immunological comparison of rye and calf histones
- 1 November 1978
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 56 (11), 1021-1027
- https://doi.org/10.1139/o78-161
Abstract
Rye and calf histones were fractionated by differential solubility and exclusion chromatography. Antibodies were produced against histones H1, H2A, H2B and H3 of both species and the extent of cross-reaction was measured by microcomplement fixation. The results allowed the identification of rye histones H2A and H2B. The near structural identity of the H3 histones was confirmed by the small immunological distance which indicated an amino acid difference of less than 2% between rye and calf. The interspecific differences found for both H2A and H2B were greater, the amino acid difference estimated from the immunological distance being between 10-20%. H1 gave no immunological cross-reaction, indicating a large (greater than 40%) difference in the structure of this protein in the 2 species. The extent of variation that a histone fraction shows between plant and animal species is probably related to its role in chromatin organization.This publication has 3 references indexed in Scilit:
- Anomalies encountered in the classification of histones. An example using wheat germ.Journal of Biological Chemistry, 1977
- Identification of histones F2b and F1 in stained gelsJournal of Chromatography A, 1976
- Chromatin structure as probed by nucleases and proteases: Evidence for the central role of hitones H3 and H4Cell, 1976