Evaluation of the Roles of Four Candida albicans Genes in Virulence by Using Gene Disruption Strains That Express URA3 from the Native Locus
Open Access
- 1 October 2003
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 71 (10), 6101-6103
- https://doi.org/10.1128/iai.71.10.6101-6103.2003
Abstract
Reintroducing URA3 to its native locus in Candida albicans not5 , not3 , bur2 , and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5′-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only NOT5 could be conclusively ascribed a role in virulence.Keywords
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