Cytokine therapy prevents left ventricular remodeling and dysfunction after myocardial infarction through neovascularization

Abstract

SPECIFIC AIMSPretreatment with a combination of granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) has been reported to attenuate left ventricular (LV) remodeling and reduce mortality after acute myocardial infarction (MI). The aim of the present study is to clarify three points: 1) whether cytokine treatment started after MI is as effective as pretreatment; 2) whether combination treatment with G-CSF and SCF is more effective than treatment with G-CSF alone; and 3) how G-CSF prevents LV remodeling and dysfunction after MI.PRINCIPAL FINDINGS1. Cytokine treatment started after MI improve mortality and cardiac functionMice were categorized into 5 groups: 1) administration of vehicle (control, n=22); 2) administration of G-CSF (100 μg/kg/day) and SCF (200 μg/kg/day) from 5 days before MI through 3 days after (pre-GS, n=16); 3) administration of G-CSF (100 μg/kg/day) and SCF (200 μg/kg/day) for 5 days after MI (post-GS, n=20); 4) administration of G-CSF (100 μg/kg/day) alone for 5 days a...