Proton-Nuclear-Magnetic-Resonance/pH-Titration Studies of the Histidines of Pancreatic Phospholipase A2
- 1 October 1979
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 100 (2), 511-518
- https://doi.org/10.1111/j.1432-1033.1979.tb04196.x
Abstract
A study using 1H NMR of the histidines of phospholipase A2 isolated from porcine, bovine and equine pancreas is reported. Assignment of histidine resonances was achieved by comparison of different enzymes and use of paramagnetic probes. pH titration curves for various histidyl resonances were obtained and compared in the presence and absence of Ca. Ca lowers the pKa of the active site histidine. NMR results are compared with the known X-ray 3-dimensional structure for the bovine enzyme.This publication has 19 references indexed in Scilit:
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