Acetyl-Coenzyme A carboxylase. Role of the prosthetic group in enzyme polymerization
- 1 March 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 145 (3), 545-548
- https://doi.org/10.1042/bj1450545
Abstract
Apo-(acetyl-CoA carboxylase) completely free from the holoenzyme was prepared from biotin-deficient rat adipose tissue by using affinity chromatography. The apoenzyme does not aggregate under conditions favouring the transition of the holoenzyme to the polymeric form. Such transition is possible after the conversion of the apoenzyme into the holoenzyme in vitro, thus demonstrating the requirement of the prosthetic biotinyl group for enzyme activation.Keywords
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