Reverse phase high speed liquid chromatography of antibiotics. II. Use of high efficiency small particle columns.

Abstract

Improved methods for separation and quantitation of cephalosporins, penicillins, tetracyclines and several miscellaneous antibiotics by reverse phase high speed liquid chromatography are presented. The methods were improved significantly by substitution of high efficiency, small particle (.apprx. 10 .mu.m) reverse phase columns in place of the previously used medium efficiency, pellicular columns. Conditions and procedures described illustrate that considerable improvements in separation and sensitivity of detection of antibiotics are achieved. Pure compounds, complex mixtures of antibiotics in a variety of dosage forms and fermentation broths are routinely analyzed by the described procedures.