Selective killing of normal or neoplastic B cells by antibodies coupled to the A chain of ricin.

Abstract

Highly specific antibodies (affinity-purified or hybridoma) directed against cell surface Ig on normal or neoplastic murine B lymphocytes were covalently coupled to the A chain of the plant toxin ricin. Such conjugates containing antibodies specific for IgM, for either of the 2 allotypes of IgD, or for the idiotype of the B cell tumor BCL1 rapidly bound in vitro to cells expressing the corresponding surface antigen and inhibited protein synthesis in such cells. A chain-coupled anti-idiotype antibody may be useful as a tumor-specific cytotoxic agent.