Ion channels in human macrophages compared with the U-937 cell line
- 1 February 1987
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 96 (1), 57-64
- https://doi.org/10.1007/bf01869334
Abstract
The human cell line U-937 has been used extensively to model many macrophage functions. We have examined the cell membranes of human monocyte-derived macrophages (HMDM) and U-937 cells to compare membrane properties as expressed by single ion channel currents. The patch-clamp technique was applied to isolated, nonactivated, inside-out patches of cell membranes obtained from HMDM and from the U-937 cell line. Voltage-gated potassium channels of similar conductance but different kinetics are present in both types of cells, and a calcium-activated potassium channel is present only in the HMDM. These differences in ion channel properties suggest fundamentally different behavior between these two cell types at the level of the cell membrane.This publication has 36 references indexed in Scilit:
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