Profibrinolysin, Antifibrinolysin, Fibrinogen and Urine Fibrinolytic Factors in the Human Subject1

Abstract

Methods for quantitation of human profibrinolysin and for semi-quantitation of urine fibrinolytic factors are described. Profibrinolysin was isolated through treatment with protamine sulfate, dilution and pH alteration. After activation with streptokinase, fibrinolytic activity was measured by the rate of lysis of a fibrin clot. Fibrinolytic activity of urine was also measured by the rate of lysis of a fibrin clot following removal of salts by dialysis. Utilizing these assays with assays previously described for antifibrinolysis and fibrinogen, it was found that plasma profibrinolysin, antifibrinolysin, and fibrinogen were significantly increased in patients with neoplasms and in pregnant women when compared to the mean concentrations of these factors in normal individuals: the increase of these 3 factors being greater in pregnant women than in patients with neoplasia. Dialyzed urine from patients with neoplasia tended to be more fibrinolytic than urine from healthy individuals.