Chicken homolog of the mos proto-oncogene.
Open Access
- 1 February 1988
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 8 (2), 923-929
- https://doi.org/10.1128/mcb.8.2.923
Abstract
We compared the sequence and properties of the chicken mos homolog with the previously characterized mouse and human c-mos genes. Sequence analysis revealed one major open reading frame of 1,047 base pairs encoding a protein of 349 amino acids. Both the nucleotide sequence and the deduced amino acid sequence showed 62% overall homology to mouse and human c-mos, but regions of higher conservation (approximately 70%) occurred in the putative ATP-binding and kinase domains. We detected mos transcripts by Northern (RNA) analyses in RNA prepared from chicken and quail ovaries and testes. Evidence for low levels of mos RNA expression in adult chicken heart, kidney, and spleen and in the entire embryo was obtained by S1 nuclease protection experiments. In contrast to the low transforming efficiency of human c-mos when linked to a mouse retroviral long terminal repeat element, chicken c-mos transformed NIH 3T3 cells as efficiently as mouse c-mos did. We also show that chicken primary embryo fibroblasts were morphologically altered when infected with an avian retroviral vector containing the chicken c-mos coding region.This publication has 42 references indexed in Scilit:
- Sequences Upstream from the Mousec-mosOncogene May Function as a Transcription Termination SignalDNA, 1986
- Analysis of the transforming potential of the human homolog of mosCell, 1986
- Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomesCell, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Modelling the ATP‐binding site of oncogene products, the epidermal growth factor receptor and related proteinsFEBS Letters, 1984
- Activation of the Transforming Potential of a Normal Cell Sequence: A Molecular Model for OncogenesisScience, 1981
- Properties of a Normal Mouse Cell DNA Sequence (sarc) Homologous to the src Sequence of Moloney Sarcoma VirusScience, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975