LYSATES OF TURKEY-GROWN PASTEURELLA-MULTOCIDA - PARTIAL SOLUBILIZATION OF THE CROSS-PROTECTION FACTOR(S)
- 1 January 1982
- journal article
- research article
- Vol. 43 (10), 1781-1785
Abstract
A lysate of turkey-grown P. multocida was treated to determine the nature of a cross-protection factor (CPF). Complete lysate was treated with proteolytic enzymes and mild heat. Cross protection was not induced with pepsin-treated lysate, but 50% cross protection was induced with trypsin-treated lysate and 100% cross protection was induced in turkeys vaccinated with lysate heated at 56.degree. C for 1 h. Complete lysate was also fractionated by differential and sucrose density gradient centrifugation. After the lysate was centrifuged for 1 h at 100,000 .times. g, the CPF was found in the supernatant and pellet fractions, indicating that the CPF was dispersed throughout; 84% of the lysate protein was in the supernatant and 14.7% of the protein in the pellet. After sucrose density gradient centrifugation, 100% cross protection was induced with the top and bottom gradient fractions and 60% with the middle fraction. Although the differential centrifugation pellet and bottom sucrose gradient fraction had protein that was disproportionately small when compared with the soluble portions of the whole lysate, they had the CPF equal in immunizing capacity.This publication has 7 references indexed in Scilit:
- LYSATES OF TURKEY-GROWN PASTEURELLA-MULTOCIDA - PROTECTION AGAINST HOMOLOGOUS AND HETEROLOGOUS SEROTYPE CHALLENGE EXPOSURES1981
- Modulation of Cross-Protection Factor(s) of Avian Pasteurella multocidaAvian Diseases, 1979
- Fowl Cholera: Cross-Protection Induced by Pasteurella multocida Separated from Infected Turkey BloodAvian Diseases, 1979
- A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of gram-negative bacteriaAnalytical Biochemistry, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Fowl Cholera: Comparison of Serologic and Immunogenic Responses of Chickens and TurkeysAvian Diseases, 1965
- Studies on Pasteurellosis. V. Two Immunogenic Types of Pasteurella multocida Associated with Fowl CholeraAvian Diseases, 1962