Separation of Vitamin B12 Analogues by High Performance Liquid Chromatography

Abstract
Good separation of Vitamin B12 analogs (mecobalamin, cobamide, cyanocobalamin and hydroxocobalamin) by reversed phase high performance liquid chromatography was studied using a 37% methanol-0.04 M tartaric acid-Na2HPO4 buffer (pH 3.0) mixture as mobile phase.