Polarity of regeneration in excised leaves of Crassula argentea. II. Auxin transport inhibitors and auxin–cytokinin interaction

Abstract

Two auxin transport inhibitors, triodobenzoic acid and 3,3a-dihydro-2-(p-methoxyphenyl)-8H-pyrazol(5,1-a)isoindol-8-one, inhibited the number of plantlets regenerated from leaves of Crassula argentea. This inhibition can be partially overcome with indole 3-acetic acid. The antiauxin, parachlorophenoxyisobutyric acid, reduced the amount of auxin-induced regeneration for 14 days in culture. High concentrations of isopentenyl adenine inhibited the auxin-induced regeneration response, but not the reduction in polarity of plantlet regeneration caused by exogenous auxin.