Near-Infrared Acousto-Optic Filtered Spectroscopic Microscopy: A Solid-State Approach to Chemical Imaging
Open Access
- 1 April 1992
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 46 (4), 553-559
- https://doi.org/10.1366/0003702924125032
Abstract
A new instrumental approach for performing spectroscopic imaging microscopy is described. The instrument integrates an acousto-optic tunable filter (AOTF) and charge-coupled-device (CCD) detector with an infinity-corrected microscope for operation in the visible and near-infrared (NIR) spectral regions. Images at moderate spectral resolution (2 nm) and high spatial resolution (1 μim) can be collected rapidly. Data are presented containing 128 × 128 pixels, although images with significantly larger formats can be collected in approximately the same time. In operation, the CCD is used as a true imaging detector, while wavelength selectivity is provided by using the AOTF and quartz tungsten halogen lamp to create a tunable source. The instrument is entirely solid state, containing no moving parts, and can be readily configured for both absorption and reflectance spectroscopies. We present visible absorption spectral images of human epithelial cells, as well as NIR vibrational absorption images of a hydrated phospholipid suspension, to demonstrate the potential of the technique in the study of biological materials. Extensions and future applications of this work are discussed.Keywords
This publication has 11 references indexed in Scilit:
- Infrared Micro-Imaging of Atherosclerotic ArteriesApplied Spectroscopy, 1991
- Multichannel Hadamard Transform Raman MicroscopyApplied Spectroscopy, 1990
- Near-infrared Fourier-transform Raman spectroscopy using fiber-optic assembliesAnalytical Chemistry, 1988
- Imaging with Functional Group Maps Using Infrared MicrospectroscopyApplied Spectroscopy, 1988
- Rapid scanning fluorescence spectroscopy using an acousto-optic tunable filterReview of Scientific Instruments, 1987
- Video MicroscopyPublished by Springer Nature ,1986
- Imaging Spectrometry for Earth Remote SensingScience, 1985
- Noncollinear acousto-optic filter with large angular apertureApplied Physics Letters, 1974
- Optimum crystal orientation for acoustically tuned optical filtersJournal of the Optical Society of America, 1974
- Acousto-Optic Tunable Filter*Journal of the Optical Society of America, 1969