Vitamin and Amino Acid Requirements ofPediococcus soyaeandPediococcus acidilacticiKitahara’s Strain

Abstract

Nutritional requirements of three strains of Ped. soyae, one strain of Ped. acidilactici (Kitahara’s strain) were determined from a complete synthetic medium. Upon inspection by the single omission method, Ped. soyae required glycine-betaine, uracil, riboflavine, pyridoxine or pyridoxal, pantothenic acid, nicotinic acid, leucovorin, biotin, glutamic acid, arginine, histidine, isoleucine, leucine, phenyl alanine, valine, tryptophane, methionine, cystine and serine with exception of the P-factor. In addition to the above mentioned nutrients, a representative strain of the organism required xanthine, folic acid, thiamine, aspartic acid and threonine for the minimum synthetic medium. Ped. acidilactici Kitahara’s strain required riboflavine, pyridoxine, pantothenic acid, nicotinic acid, biotin and all components of the basal medium consisting of amino acids except methionine, but it did not require leucovorin, glycine-betaine and organic bases. Nutritional requirements of Ped. pentosaceus, Kitahara’s strain was proved to be quite identical with Ped. cerevisiae Pederson (= Ped. pentosaceus Mees).