A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Transactions of the Royal Society of Tropical Medicine and Hygiene
- Vol. 78 (5), 622-624
- https://doi.org/10.1016/0035-9203(84)90221-9
Abstract
Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 109 erythrpcytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1·081 and 1·091 g cm−3, depending on the maturity of the p arasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.Keywords
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