Correlation of cell-cycle kinetics, hormone receptors, histopathology, and nodal status in human breast cancer
- 15 October 1984
- Vol. 54 (8), 1586-1590
- https://doi.org/10.1002/1097-0142(19841015)54:8<1586::aid-cncr2820540820>3.0.co;2-9
Abstract
DNA ploidy and percent of (%S-phase) S-phase cells were determined from the DNA content distribution of 21 benign and 76 malignant (69 primary, 7 metastatic) breast tumors using flow cytometry. All of the benign tumors were diploid, whereas 89% of the malignant tumors had measurable aneuploidy. Multiple stem-lines were observed in approximately 10% of the malignant tumors. The ploidy distribution of the malignant tumors was bimodal with an increased frequency of tumors with a near diploid DNA index (DI), and a second group with DI ranging from triploid to tetraploid. The percentage of cells in S-phase ranged from <1% to 37.4%. DI and %S were significantly higher in poorly differentiated duct carcinomas, medullary carcinomas, and recurrent tumor metastases. DI and %S were also significantly higher in estrogen-receptor-negative tumors. There was no correlation between DI or %S and the extent of axillary nodal metastases. However, within the groups of node-negative and node-positive patients, DI and %S were not randomly distributed but were significantly correlated with degree of nuclear differentiation. Both parameters were higher in poorly differentiated tumors compared with well-differentiated tumors, indicating significant intrastage heterogeneity in tumor ploidy and proliferation characteristics. Determination of the prognostic significance of DI and %S will require a longer follow-up time.This publication has 13 references indexed in Scilit:
- A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysisCytometry, 1983
- Ploidy, proliferative activity and estrogen receptor content in human breast cancerCytometry, 1982
- RNA Content and Chromatin Structure in Cycling and Noncycling Cell Populations Studied by Flow CytometryPublished by Elsevier ,1982
- Estrogen Receptor Heterogeneity and the Relationship between Estrogen Receptor and the Tritiated Thymidine Labeling Index in Human Breast CancerOncology, 1982
- Flow cytometry of breast carcinoma: I. Relation of DNA ploidy level to histology and estrogen receptorCancer, 1981
- Flow cytometry of breast carcinoma: II. Relation of tumor cell cycle distribution to histology and estrogen receptorCancer, 1981
- Cellular DNA content as a marker of neoplasia in manAmerican Journal Of Medicine, 1980
- Low incidence of estrogen receptor in breast carcinomas with rapid rates of cellular replicationCancer, 1977
- Prognosis in breast cancer utilizing histologic characteristics of the primary tumorCancer, 1975
- Histological Grading and Prognosis in Breast CancerBritish Journal of Cancer, 1957