Mites and house dust allergy. IV. Antigen and allergen(s) of Dermatophagoides pteronyssinus extract
- 1 March 1975
- journal article
- Published by Wiley in Clinical and Experimental Allergy
- Vol. 5 (1), 69-77
- https://doi.org/10.1111/j.1365-2222.1975.tb01837.x
Abstract
The antigenic composition of D. pteronyssinus extract was investigated by using antisera raised in rabbits by injection of the mile extract in Freund's adjuvant. Immunodiffusion analysis revealed the presence in the mite extract of many antigenic constituents, most of which were also common to human dander and house dust extracts. Four antigenic components not shared by human dander appeared to be common to D.farinae extract. D. pteronyssinus extract was then fractionated on Sephadex G‐75 and DEAE‐Sephadex. Immunodiffusion analysis of the most active fraction revealed, after heat‐treatment, the presence of three precipitin lines, one of which appeared not to be shared by nutrient medium or by D. farinae extract constituents. When the anti‐mite rabbit serum was fixed to an immunosorbent and incubated with a house dust extract it was constantly able to induce a strong decrease or a complete loss of the allergenicity of the dust extract in man. These results suggest that mite allergen(s) are immunogenic in the rabbit and provide more direct evidence for identity of the allergens in house dust and mite.Keywords
This publication has 9 references indexed in Scilit:
- Mites and house dust allergy III. In vitro lymphocyte transformation and precipitating antibody to house dust and mite (Dermatophagoides pteronyssinus) extract in atopic and non‐atopic individualsClinical and Experimental Allergy, 1973
- The allergens responsible for house dust allergy: I. Comparison of Dermatophagoides pteronyssinus and house dust extracts by assay of histamine release from allergic human leukocytesJournal of Allergy and Clinical Immunology, 1972
- Mites and house dust allergyClinical and Experimental Allergy, 1972
- Mites and house dust allergyClinical and Experimental Allergy, 1972
- RADIOIMMUNOASSAYS EMPLOYING IMMUNOSORBENTSActa Endocrinologica, 1969
- Allergenic identity between the common floor mite (Dermatophagoides farinae Hughes, 1961) and house dust as a causative antigen in bronchial asthmaJournal of Allergy, 1968
- MITES AND HOUSE-DUST ALLERGYThe Lancet, 1968
- MITES AND HOUSE-DUST ALLERGY IN BRONCHIAL ASTHMAThe Lancet, 1968
- The house-dust mite (Dermatophagoides pteronyssinus) and the allergens it produces. Identity with the house-dust allergenJournal of Allergy, 1967