The Oxidation of Glycerol by Streptococcus faecalis

Abstract

Glycerol is oxidized by S. faecalis, Strain 24, at a rapid rate (Qo2(N) = 400 [long dash]700), the over-all reaction being: Glycerol + O2 [forward arrow] lactate + H2O2 The pathway of this oxidation involves phosphorylation. The glycerol is phosphorylated (with adenosine-5-triphos-phate) to a glycerol phosphate, which is oxidized to triose phosphate. The latter is converted into lactic acid. The oxidation is inhibited by the accumulation of H2O2 to a cone, of 0.002 M. Various properties of the enzyme system are decsribed.