Changes in Activity Levels of Certain Aminoacid-RNA Ligases during the Growth Cycle of Streptococcus thermophilus.
- 31 December 1964
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 19 (1), 191-196
- https://doi.org/10.3891/acta.chem.scand.19-0191
Abstract
The formation of enzymes which activate the amino-acids, amino-acid-ribonucleic-acid RNA ligases, was studied during the active growth periods of S. thermophilus by measuring the rate of the adenosine triphosphate ATP-(P32) pyrophosphate exchange reaction. The amino-acids isoleucine, leucine and valine were activated by the crude enzyme preparations from S. thermophilus; lysine and tryptophan were not activated. Only one maximum level of enzyme activity of the investigated amino-acid-RNA ligases was observed. This maximum occurred before the middle of the exponential phase and was in all cases some 2 or 3 times the activity at the beginning of growth.Keywords
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