Abstract
The vaccines that are used for the prophylaxis of virus and rickettsial infections usually consist of suspensions of infected animal tissues in which the virus is either killed or attenuated. These types of vaccines are necessary because of the extreme parasitism of the infectious agents, which have resisted attempts to grow them in mediums that are free of living tissue. In recent years, technics have been developed for the propagation of many viral and rickettsial agents in the tissues or fluid cavities of embryonated hens' eggs. As these technics were perfected, preparations containing the tissues or fluids of infected chick . . .