THE EFFECTS OF A CARDIAC GLYCOSIDE ON SUBCELLULAR STRUCTURES WITHIN NERVE CELLS AND THEIR PROCESSES IN SYMPATHETIC GANGLIA AND SKELETAL MUSCLE
- 1 February 1962
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 40 (2), 303-315
- https://doi.org/10.1139/o62-033
Abstract
Nerve cells and their processes in cat sympathetic ganglia and frog skeletal muscle have shown on electron microscopic examination alterations in subcellular morphology as a result of treatment with digoxin. Non-nervous cells were unaffected by the drug. These changes included, in ganglia, swelling of the affected cells, shrinkage of mitochondria with pronounced increase in internal density, swelling of Nissl substance in nerve cell bodies, and loss of structural detail in nerve processes. At the myoneural junction the motor nerve endings were swollen, mitochondria were altered, and the synaptic vesicles were reduced in numbers, those that remained being swollen. These changes were accompanied by invagination of the axon surface by Schwann cell processes.Cell swelling, but not the subcellular changes, was prevented by substitution of sulphate for chloride ions in the extracellular space. When the extracellular sodium ion concentration was reduced to 20 meq/l. the cells were completely protected against digoxin. It is concluded that swelling is caused by net uptake of sodium and chloride as a result of the known inhibitory action of digoxin on sodium extrusion by nerve cells. The possibility that these structural changes in subcellular organelles may be caused by a raised concentration of intracellular sodium ions, such as might occur during activity of excitable cells, is discussed.Keywords
This publication has 16 references indexed in Scilit:
- A study of curare action with an electrical micro-methodProceedings of the Royal Society of London. B. Biological Sciences, 1957
- The action of cardiac glycosides on sodium and potassium movements in human red cellsThe Journal of Physiology, 1957
- Factors influencing the sodium movement in frog muscle with a discussion of the mechanism of sodium movementThe Journal of Physiology, 1957
- Influence of Ouabain, Strophanthidin and Dihydrostrophanthidin on Sodium and Potassium Transport in Frog SartoriiAmerican Journal of Physiology-Legacy Content, 1956
- Potassium movements in washed erythrocytesThe Journal of Physiology, 1955
- Active transport of cations in giant axons from Sepia and LoligoThe Journal of Physiology, 1955
- THE FINE STRUCTURE OF NEURONSThe Journal of cell biology, 1955
- Die Wirkung von Desoxycorticosteron auf den aktiven Kationenaustausch an RattenblutzellenCellular and Molecular Life Sciences, 1954
- Electrical activity and intracellular sodium concentration in frog muscleThe Journal of Physiology, 1953
- Potassium accumulation in muscle and associated changes1The Journal of Physiology, 1941