Kinetochore microtubules in crane‐fly spermatocytes: Untreated, 2°C‐treated and 6°C‐grown spindles
- 1 January 1986
- journal article
- research article
- Published by Wiley in Cell Motility
- Vol. 6 (4), 428-438
- https://doi.org/10.1002/cm.970060408
Abstract
We investigated the involvement of kinetochore microtubules (kMTs) in mediating chromosome-to-pole connections in crane-fly (Nephrotoma suturalis and Nephrotoma ferruginea) spermatocytes. Two experimental treatments were used to yield spindles with reduced numbers of nonkinetochore microtubules (nkMTs). Short-term (10–15 min) exposure of spermatocytes to 2°C caused depolymerization of the majority of nkMTs, resulting in a kMT:(kMT + nkMT) ratio of 0.76. Long-term (24h) exposure to 2°C followed by recovery at 6°C resulted in a kMT:(kMT + nkMT) ratio of 0.55, the spindle having more nkMTs than a 2°C-treated spindle but fewer than an untreated spindle, in which the kMT:(kMT + nkMT) ratio was 0.27. The numbers and lengths of kMTs in 6°C-grown spindles were similar to those in untreated cells, suggesting that the overall inhibition of MT assembly at 6°C apparently did not affect the mechanism by which kMTs are formed. We observed most kMTs of early anaphase spindles to be long (>3 μm), and many extended to the polar regions of the spindle. Thus, the crane-fly spindle appears not to be as atypical as it was previously suggested to be.Keywords
This publication has 30 references indexed in Scilit:
- Spindle microtubules and their mechanical associations after micromanipulation in anaphase.The Journal of cell biology, 1982
- Meiosis in Drosophila melanogaster. II. The prometaphase-I kinetochore microtubule bundle and kinetochore orientation in males.The Journal of cell biology, 1982
- Dynamics of spindle microtubule organization: kinetochore fiber microtubules of plant endosperm.The Journal of cell biology, 1982
- Biochemical analysis of actin in crane-fly gonial cells: evidence for actin in spermatocytes and spermatids--but not sperm.The Journal of cell biology, 1980
- Functional implications of cold-stable microtubules in kinetochore fibers of insect spermatocytes during anaphase.The Journal of cell biology, 1980
- An analysis of spindle ultrastructure during anaphase of micronuclear division in TetrahymenaCell Motility, 1980
- Micromanipulation studies of chromosome movement. II. Birefringent chromosomal fibers and the mechanical attachment of chromosomes to the spindle.The Journal of cell biology, 1979
- COLD‐LABILE AND COLD‐STABLE MICROTUBULES IN THE MITOTIC SPINDLE OF MAMMALIAN CELLS*Annals of the New York Academy of Sciences, 1975
- Mitosis in the cellular slime mold Polysphondylium violaceum.The Journal of cell biology, 1975
- Birefringence and fine structure of spindles in spermatocytes of Nephrotoma suturalis at metaphase of first meiotic divisionJournal of Ultrastructure Research, 1974