Use of Inhibitors for Selective Isolation and Enumeration of Cytophagas from Natural Substrates

Abstract

Several antibacterial agents were tested against a range of cytophagas and other bacteria representing types commonly found in soil and marshy places. From these tests 2 antibiotics (penicillin 15 units/ml and chloramphenicol 5.0 [mu]g/ml) and 2 bile salts (sodium taurocholate 8.0 mg/ml and sodium cholate 6.0 mg/ml) were selected for incorporation into alkaline enrichment media to achieve selective growth of cytophagas. This mixture of antibiotics and bile salts was tested with a number of soils and its efficiency compared with several other methods for selective development of cytophagas; bile salts inhibited 2 common species. The most suitable mixture for the selection of cytophagas was enrichment medium with penicillin and chloramphenicol.