Liver collagen synthesis in murine schistosomiasis.
Open Access
- 1 April 1977
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 59 (4), 666-674
- https://doi.org/10.1172/jci108685
Abstract
Collagen synthesis was measured in liver slices obtained from mice with hepatosplenic schistosomiasis. Enlarged fibrotic livers from these mice contained 20 times more collagen than normal. This model of hepatic fibrosis results from an inflammatory granulomatous host response to Schistosoma mansoni ova in portal tracts, rather than from direct lover cell injury as with carbon tetrachloride-induced liver fibrosis. Collagen synthesis, as measured by the formation of labeled protein-bound hydroxyproline, occurred in granulomas isolated from fibrotic livers. Labeled collagen that cochromatographed with type I collagen was extracted with neutral salt solution from liver slices incubated with labeled proline. The free proline pool of the liver was doubled in infected mice; coordinately, liver slices from these animals showed maximal collagen production when the concentration of free proline in the medium was raised to 0.4 mM, the same level measured in the fibrotic livers. Under such conditions, collagen synthesis was at a rate equivalent to the formation of 5.4 nmol of protein-bound hydroxyproline per g liver in 6 h. In comparative incubations in medium containing 0.2 mM proline, fibrotic liver slices produced 16-fold more collagen than normal slices. The proline analogue, L-azetidine 2-carboxylic acid, effectively inhibited synthesis of labeled collagen by fibrotic liver slices. These studies show the synthesis of collagen in a reproducible animal model of the most prevalent form of human liver fibrosis. Difinitition of the controlling factors in this system is of interest for the general problem of fibrosis produced by immunological responses.This publication has 28 references indexed in Scilit:
- Lymphokine stimulation of collagen accumulation.JCI Insight, 1976
- Native collagen formation by liver parenchymal cells in cultureNature, 1976
- The Secretion of Migration Inhibitory Factor by Intact Schistosome Egg Granulomas maintained in vitroNature, 1973
- Inhibition of Liver Fibrosis by L-Azetidine-2-Carboxylic Acid in Rats Treated with Carbon TetrachlorideJCI Insight, 1973
- Regulation of the Prevalence and Intensity of Schistosomiasis in Man: Immunology or Ecology?The Journal of Infectious Diseases, 1973
- Isolation and characterization of a chick cartilage collagen containing three identical chainsBiochemistry, 1971
- Compartmental analysis of collagen synthesis in fetal rat calvaria: I. Perturbations of proline transportBiochimica et Biophysica Acta (BBA) - General Subjects, 1971
- Amino acid transport in boneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1967
- An experimental study of the pathogenesis and reversibility of schistosomal hepatic fibrosisThe Journal of Pathology and Bacteriology, 1964
- OBSERVATIONS ON AMINO ACID METABOLISM OF SCHISTOSOMA MANSONI IN A CHEMICALLY DEFINED MEDIUMAnnals of the New York Academy of Sciences, 1963