Phosphate transport in ehrlich ascites tumor cells and the effect of arsenate
- 1 February 1972
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 79 (1), 73-77
- https://doi.org/10.1002/jcp.1040790108
Abstract
The effect of extracellular Pi and arsenate on Pi‐transport in Ehrlich ascites tumor cells has been studied. Pi‐transport can be described by Michaelis‐Menten kinetics; the maximal flux equal to 44 mmoles (kg cell water)−1 hour−1 and Km equal to 3.3 × 10−4 M. Arsenate is a competitive inhibitor of Pi‐transport with an inhibition constant (Ki) equal to 2.41 × 10−3 M.The data support the hypothesis that cellular Pi is regulated by the cell membrane through the mediation of a carrier system.Keywords
This publication has 10 references indexed in Scilit:
- Lanthanum-Induced alterations in cellular electrolytes and membrane potential in ehrlich ascites tumor cellsJournal of Cellular Physiology, 1972
- Phosphorus incorporation in the ehrlich ascites tumor cellJournal of Cellular Physiology, 1971
- Steady-state distribution of phosphate across the membrane of the Ehrlich ascites tumor cellBiochimica et Biophysica Acta (BBA) - Biomembranes, 1970
- Scintillation counting of 32P without added scintillator in aqueous solutions and organic solvents and on dry chromatographic mediaAnalytical Biochemistry, 1970
- Phosphate partition and transport in the Ehrlich mouse ascites tumor cellBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966
- The substrate-facilitated transport of the glucose carrier across the human erythrocyte membraneBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1965
- Control Mechanisms of Glycolysis in Ehrlich Ascites Tumor CellsJournal of Biological Chemistry, 1965
- The role of Pi in the control of glycolysis in ascites tumor cellsBiochemical and Biophysical Research Communications, 1965
- Interactions of Arsenate with the Phosphate-Transporting System of YeastThe Journal of general physiology, 1963
- THE COLORIMETRIC DETERMINATION OF PHOSPHORUSJournal of Biological Chemistry, 1925