Real-Time Imaging of Tunable Adenosine 5-Triphosphate Release from an MCM-41-Type Mesoporous Silica Nanosphere-Based Delivery System

Abstract
We studied a mesoporous silica nanosphere (MSN) material with tunable release capability for drug delivery applications. We employed luciferase chemiluminescence imaging to investigate the kinetics and mechanism of the adenosine 5-triphosphate (ATP) release with various disulfide-reducing agents as uncapping triggers. ATP molecules were encapsulated within the MSNs by immersing dry nanospheres in aqueous solutions of ATP followed by capping of the mesopores with chemically removable caps, such as cadmium sulfide (CdS) nanoparticles and poly(amido amine) dendrimers (PAMAM), via a disulfide linkage. By varying the chemical nature of the “cap” and “trigger” molecules in our MSN system, we discovered that the release profiles could indeed be regulated in a controllable fashion.