ISOLATION AND PARTIAL CHARACTERIZATION OF BSTVI, A THERMOSTABLE ISOSCHIZOMER OF XHOI
- 1 January 1985
- journal article
- research article
- Vol. 10 (4), 655-662
Abstract
A type II restriction endonuclease, which was named BstVI, was isolated and partially purified from a spore-forming, gram-positive thermophilic bacilli [Bacillus Steorothermophilus]. On the basis of its digestion patterns on various DNA, this enzyme is an isoschizomer of XhoI, isolated originally from Xanthomonas holcicola. Besides being highly thermostable, the enzyme is produced in very large amounts by this bacterial strain. A single purification step renders it free of unspecific nucleases and suitable for performing restriction analysis and cloning experiments.This publication has 8 references indexed in Scilit:
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