The Chloroplast Envelope ofPhaseolus vulgarisL.

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Abstract
An isolation procedure for the chioroplast envelope of Phaseolus vulgaris L. is described. A carotenoid-enriched membrane fraction was obtained from purified chioroplasts using hypotonic shock followed by sucrose density gradient centrifugation. Chlorophyll was almost absent from the isolated membranes. Total lipid, fatty acid, and protein analyses of the fraction suggested that it possessed properties closely analogous to some of those previously reported for chloroplast envelopes of other species.