Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants
- 1 April 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 130 (1), 4-10
- https://doi.org/10.1128/jb.130.1.4-10.1977
Abstract
E. coli K-12 hisT mutants were isolated and their properties were studied. These mutants are derepressed for the histidine operon, map close to the purF locus at .apprx. 49.5 min on the E. coli linkage map and lack pseudouridylate synthetase activity. The defect in this enzyme leads to the absence of pseudouridines in the anticodon loop of several tRNA species, as evidenced by the altered elution profile on reversed-phase chromatography and resistance to amino acid analogs. The hisT mutants studied have a reduced growth rate that appears to be linked to hisT, although it is not known whether it is due to the same mutation. The normal generation time can be restored by supplementing the medium with adenine, uracil and isoleucine.This publication has 39 references indexed in Scilit:
- TRANSFER RNA INVOLVEMENT IN THE REGULATION OF ENZYME SYNTHESISAnnual Review of Microbiology, 1975
- Regulation of the expression of the histidine operon in Salmonella typhimuriumNature, 1974
- Overproduction of hisH and hisF gene products leads to inhibition of cell division in SalmonellaCanadian Journal of Microbiology, 1972
- The nucleotide sequence of two leucine tRNA species from Escherichia coli K12Biochemical and Biophysical Research Communications, 1971
- Improved separation of transfer RNA's on polychlorotrifluoroethylene-supported reversed-phase chromatography columnsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- The Nucleotide Sequence of Methionine Transfer RNAMEuropean Journal of Biochemistry, 1970
- The sequence of phenylalanine tRNA from E. coliFEBS Letters, 1969
- Transcription during bacteriophage T4 development: Synthesis and relative stability of early and late RNAJournal of Molecular Biology, 1968
- Histidine regulatory mutants in Salmonella typhimuriumJournal of Molecular Biology, 1966
- Effect of α-methylhistidine on the control of histidine synthesisJournal of Molecular Biology, 1964