Improvement in HLA‐DQB typing by PCR‐RFLP: Introduction of a constant restriction site in one of the primers for digestion control
- 1 August 1992
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 40 (2), 86-89
- https://doi.org/10.1111/j.1399-0039.1992.tb01964.x
Abstract
The PCR-RFLP method previously reported by Inoko is a powerful technique for HLA class II typing. The reliability of RFLP interpretation depends on complete digestion by restriction endonucleases using a modified primer with restriction sites as an internal digestion control. The use of restriction enzymes which recognize specific HLA DQB allelic variations makes HLA DQB genotyping possible.Keywords
This publication has 15 references indexed in Scilit:
- Rapid typing of HLA-DQB1 DNA polymorphism using nonradioactive oligonucleotide probes and amplified DNAImmunogenetics, 1991
- Nomenclature for Factors of the HLA system, 1990Tissue Antigens, 1991
- HLA Class II typing by digestion of PCR‐amplified DNA with allele‐specific restriction endonucleases will fail to unequivocally identify the genotypes of many homozygous and heterozygous individualsTissue Antigens, 1990
- PCR‐RFLP method holds great promise for complete HLA class II genotypingTissue Antigens, 1990
- A simple and rapid method for HLA-DP genotyping by digestion of PCR-amplified DNA with allele-specific restriction endonucleasesHuman Immunology, 1990
- A simple and rapid method for HLA‐DRB and ‐DQB typing by digestion of PCR‐amplified DNA with allele specific restriction endonucleasesTissue Antigens, 1990
- A simple and rapid method for HLA‐DQA1 genotyping by digestion of PCR‐amplified DNA with allele specific restriction endonucleasesTissue Antigens, 1989
- A Molecular Basis for MHC Class II—Associated AutoimmunityScience, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Transplantation ImmunologyNew England Journal of Medicine, 1987