Moloney murine sarcoma virions synthesize full-genome-length double-stranded DNA in vitro.
- 1 July 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (7), 3294-3298
- https://doi.org/10.1073/pnas.76.7.3294
Abstract
Moloney murine sarcoma virus (MSV) virions incubated under optimal conditions support extensive synthesis of double-stranded DNA. The major product, a 5950-base-pair (6-kilobase-pair DNA) double-stranded DNA, was characterized by cleavage with [Hae II, HindIII] restriction endonucleases and contained a 600-nucleotide-long direct repeat at both ends of the MSV genome. Linear DNA molecules made in vivo shortly after infection were compared to the linear double-stranded DNA synthesized in vitro. The restriction maps of both viral DNA products were indistinguishable. The 600-base-pair repeat results in a progeny DNA molecule that is longer than the parental MSV genomic RNA. The generation of this repeat must involve a mechanism that allows the viral reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) to copy 5''- and 3''-terminal genomic (+) strand sequences twice.This publication has 33 references indexed in Scilit:
- In vitro synthesis of a 9 kbp terminally redundant DNA carrying the infectivity of moloney murine leukemia virusCell, 1979
- A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfectionCell, 1979
- Mapping unintegrated avian sarcoma virus DNA: Termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNACell, 1978
- Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sitesCell, 1978
- Mapping of linear and circular forms of mouse mammary tumor virus DNA with restriction endonucleases: evidence for a large specific deletion occurring at high frequency during circularization.Proceedings of the National Academy of Sciences, 1978
- Kinetics of synthesis, structure and purification of avian sarcoma virus-specific DNA made in the cytoplasm of acutely infected cellsJournal of Molecular Biology, 1978
- Analysis of a 5′ leader sequence on murine leukemia virus 21S RNA: Heteroduplex mapping with long reverse transcriptase productsCell, 1978
- In vitro synthesis of infectious DNA of murine leukaemia virusNature, 1977
- Rous sarcoma virus genome is terminally redundant: the 3' sequence.Proceedings of the National Academy of Sciences, 1977
- Rous sarcoma virus genome is terminally redundant: the 5' sequence.Proceedings of the National Academy of Sciences, 1977