Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines
- 9 April 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 281 (1-2), 188-190
- https://doi.org/10.1016/0014-5793(91)80390-o
Abstract
Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25°C or 37°C caused the release clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride) (10 mM), chloroquin (0.1 mM) and monodansyleadaverine (10 mM) significantly reduced the extent of dissociation.Keywords
This publication has 18 references indexed in Scilit:
- Effects of cytoplasmic acidification on clathrin lattice morphology.The Journal of cell biology, 1989
- Herpes simplex virus type 1 penetration initiates mobilizaton of cell surface proteinsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1988
- Comparison of the interaction of the anti-viral chemotherapeutic agents amantadine and tromantadine with model phospholipid membranesBioscience Reports, 1987
- Interaction of basic compounds with coated vesiclesBiochemistry, 1984
- Inhibition of Semliki Forest Virus Penetration by Lysosomotropic Weak BasesJournal of General Virology, 1982
- Effect of basic compounds on the polymerization of clathrinBiochemistry, 1981
- Transglutaminase is essential in receptor-mediated endocytosis of α2-macroglobulin and polypeptide hormonesNature, 1980
- Amines inhibit the clustering of α2-macroglobulin and EGF on the fibroblast cell surfaceNature, 1979
- Coated vesicles from pig brain: Purification and biochemical characterizationJournal of Molecular Biology, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970