The Polyclonal B Cell Activator Dextran-Sulphate Induces Formation of Colony Stimulating Activity
- 1 April 1978
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 7 (4), 277-284
- https://doi.org/10.1111/j.1365-3083.1978.tb00455.x
Abstract
The effect of dextran-sulfate (DS), a polyclonal activator known to stimulate immature murine B[bone marrow-derived]-cells, was assayed in a culture system allowing the growth of myeloid cells. DS induced the production of a myeloid colony stimulating factor (CSF) by cells from spleen and bone marrow. Nylonwool purified mouse spleen cells, enriched for T[thymus-derived]-cells, showed a diminished CSF production in response to DS; CSF production in response to Con A [concanavalin A] was increased. DS induced CSF in spleen and bone marrow cells from nude mice. Removal of macrophages did not affect CSF production. The CSF induced was non-dialysable and no small MW or lipoprotein inhibitors could be demonstrated. DS probably activates cells other than T cells or macrophages (possibly B cells or null cells) to produce a myeloid stem cell stimulating substance. Interactions between lymphoid and myeloid cells may take place during differentiation.This publication has 21 references indexed in Scilit:
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