Image analysis of restriction enzyme fingerprint autoradiograms
- 1 April 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Bioinformatics
- Vol. 5 (2), 101-106
- https://doi.org/10.1093/bioinformatics/5.2.101
Abstract
A genome mapping system has been developed that reads and assembles data from clones analysed by restriction enzyme fragmentation and polyacrylamide gel electrophoresis. Input data for the system can be most effectively obtained by the use of a scanning densitometer and image-processing package, such as that described in this article. The image-processing procedure involves preliminary location of bands, cooperative tracking of lanes by correlation of adjacent bands, a precise densitometric pass, alignment of the marker bands with the standard, optional interactive editing, and normalization of the accepted bands.This publication has 4 references indexed in Scilit:
- Software for genome mapping by fingerprinting techniquesBioinformatics, 1988
- A non-radioactive automated method for DNA sequence determinationJournal of Biochemical and Biophysical Methods, 1986
- Toward a physical map of the genome of the nematode Caenorhabditis elegansProceedings of the National Academy of Sciences, 1986
- Fluorescence detection in automated DNA sequence analysisNature, 1986