Regulation by cAMP and vasoactive intestinal peptide of phosphorylation of specific proteins in striatal cells in culture.
- 1 October 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (20), 7790-7794
- https://doi.org/10.1073/pnas.85.20.7790
Abstract
We have studied three low molecular weight phosphoproteins, ARPP-16, ARPP-19, and ARPP-21 (cAMP-regulated phosphoproteins of Mr 16,000, 19,000, and 21,000, respectively) in reaggregate cultures from various regions of fetal mouse brain. ARPP-16 and ARPP-16 and ARPP-21 were detected only in striatal and cortical cultures. In contrast, ARPP-19, which is structurally related to ARPP-16, was also present in reaggregate cultures prepared from thalamus and ventral and dorsal mesencephalon, as well as in monolayer cultures of astroglial cells. In striatal aggregates cultured over a 3-week period, the relative levels of ARPP-16, ARPP-21, and synapsin I/protein IIIa (synaptic vesicle-associated phosphoproteins closely related to each other and treated as a single entity in the present study) increased with time, whereas the level of ARPP-19 decreased. Incubation of striatal aggregates with 8-Br-cAMP, forskolin, or vasoactive intestinal peptide increased the phosphorylation of two proteins enriched in specific neurons (ARPP-16 and ARPP-21) and two more widely distributed proteins (ARPP-19 and synapsin I/protein IIIa) is regulated by cAMP and vasoactive intestinal peptide in striatal cells in culture. These phosphoproteins may therefore play a role in mediating some of the actions of vasoactive intestinal peptide in the caudate-putamen.Keywords
This publication has 25 references indexed in Scilit:
- Noradrenaline- and vasoactive intestinal peptide-containing neuronal systems in neocortex: Functional convergence with contrasting morphologyNeuroscience, 1988
- Morphology of striatal neurons containing VIP‐like immunoreactivityJournal of Comparative Neurology, 1987
- Biochemical and morphological studies on GABA neurons in reaggregate cultureBrain Research, 1987
- Synapsin I: A synaptic vesicle-associated neuronal phosphoproteinBiochemical Pharmacology, 1986
- Vasoactive intestinal polypeptide increases inositol phospholipid breakdown in the rat superior cervical ganglionBrain Research, 1986
- Nerve growth factor promotes cholinergic development in brain striatal cultures.Proceedings of the National Academy of Sciences, 1985
- Depolarizing agents and cyclic nucleotides regulate the phosphorylation of specific neuronal proteins in rat cerebral cortex slices.Proceedings of the National Academy of Sciences, 1978
- Effect of vasoactive intestinal peptide (VIP) and other peptides on cAMP accumulation in rat brainBiochemical Pharmacology, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970