Synthesis of DNA in Toluene-Treated Chlamydomonas reinhardi
- 1 February 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (2), 490-494
- https://doi.org/10.1073/pnas.69.2.490
Abstract
Toluene-treated Chlamydomonas reinhardi incorporate deoxynucleoside triphosphates (dNTPs) into DNA. Incorporation requires all four dNTPs, but requires neither ATP nor added DNA. The incorporation reaction is linear for nearly 20 min. The product of synthesis in treated cells is mainly chloroplast (beta-component) DNA. However, a small quantity of nuclear (alpha-component) DNA is also synthesized. Synchronously grown cells are most efficient in dNTP incorporation at a time in the cell cycle when chloroplast DNA is normally replicated. Toluene treatment disrupts the internal morphology of the cell, but leaves the outer membrane of the chloroplast intact.Keywords
This publication has 9 references indexed in Scilit:
- Analysis of DNA synthesis during meiotic prophase in LiliumJournal of Molecular Biology, 1971
- Replication and Repair of DNA in Cells of Escherichia coli Treated with TolueneProceedings of the National Academy of Sciences, 1970
- On the formation of a homogeneous zygotic population in Chlamydomonas reinhardtiiDevelopmental Biology, 1970
- Replication of chloroplast DNA in Chlamydomonas reinhardi during vegetative cell cycle: its mode and regulation.Proceedings of the National Academy of Sciences, 1967
- Deoxyribonucleic acid replication in meiosis of Chlamydomonas reinhardiJournal of Molecular Biology, 1967
- Evidence for repair-replication of ultraviolet damaged DNA in bacteriaJournal of Molecular Biology, 1964
- The control of gametic differentiation in liquid cultures of ChlamydomonasJournal of Cellular and Comparative Physiology, 1964
- METABOLICALLY LABILE DEOXYRIBONUCLEIC ACIDProceedings of the National Academy of Sciences, 1963
- MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDIProceedings of the National Academy of Sciences, 1960