A New Liquid Medium for Trypanosoma (Schizotrypanum) cruzi*
- 1 May 1975
- journal article
- Published by Wiley in The Journal of Protozoology
- Vol. 22 (2), 265-270
- https://doi.org/10.1111/j.1550-7408.1975.tb05864.x
Abstract
A new liquid medium, with fetal calf serum as the sole undefined component, was devised for the cultivation of Trypanosoma cruzi. The need for the serum is ascribed to its mitogenic proteins, which stimulate division of, and the uptake of [3-H-] thymidine by the parasites. In the new medium, T. cruzi has a cycle culminating in the apperance of up to 90 per cent metacyclic forms in the stationary phase. This cycle is repeated on each serial transfer.Keywords
This publication has 8 references indexed in Scilit:
- Nutrition and Biosynthetic Capabilities of Flagellates: Problems of in vitro Cultivation and DifferentiationPublished by Wiley ,1974
- Cultivation and Morphogenesis of Trypanosoma cruzi in Improved Liquid Media1The Journal of Protozoology, 1971
- Buffer Combinations for Mammalian Cell CultureScience, 1971
- Preparation and properties of nuclear and satellite deoxyribonucleic acid of Trypanosoma cruziJournal of Molecular Biology, 1967
- Stearic acid, an Essential Growth Factor for Trypanosoma cruziJournal of General Microbiology, 1963
- Cultural and Physiological Observations on Trypanosoma rhodesiense and Trypanosoma gambienseJournal of Parasitology, 1950
- Nova tripanozomiaze humana: estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n. sp., ajente etiolojico de nova entidade morbida do homemMemórias do Instituto Oswaldo Cruz, 1909