Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium.
- 1 December 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (23), 8530-8534
- https://doi.org/10.1073/pnas.84.23.8530
Abstract
We determined the complete nucleotide sequence of the 6354-base-pair plasmid pHV2 of the archaebacterium Halobacterium volcanii. This plasmid is present in approximately six copies per chromosome. We have generated a strain, H. volcanii WFD11, cured of pHV2 by treatment of liquid cultures with ethidium bromide. We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro.This publication has 33 references indexed in Scilit:
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencingGene, 1984
- [2] New M13 vectors for cloningMethods in Enzymology, 1983
- Unusual physical organization of the Halobacterium genomeNature, 1982
- The enzymatic preparation of [α-32P]nucleoside triphosphates, cyclic [32P]AMP, and cyclic [32P]GMPBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
- ArchaebacteriaJournal of Molecular Evolution, 1978
- Phylogenetic structure of the prokaryotic domain: The primary kingdomsProceedings of the National Academy of Sciences, 1977
- Computer analysis of nucleic acid regulatory sequences.Proceedings of the National Academy of Sciences, 1977
- Halobacterium volcanii spec. nov., a Dead Sea halobacterium with a moderate salt requirementArchiv für Mikrobiologie, 1975
- FURTHER CHARACTERIZATION OF PARTICULATE FRACTIONS FROM LYSED CELL ENVELOPES OF HALOBACTERIUM HALOBIUM AND ISOLATION OF GAS VACUOLE MEMBRANESThe Journal of cell biology, 1968