An inhibitor of mammalian collagenase active at micromolar concentrations from an Actinomycete culture broth.
- 1 January 1987
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 40 (12), 1757-1761
- https://doi.org/10.7164/antibiotics.40.1757
Abstract
Anti-collagenase activity was detected in the culture supernatant of an Actinomycete strain S 4373. The molecule was purified by solvent extraction, medium pressure and high pressure reverse phase chromatography and finally by HPLC gel filtration. The pure product was analyzed by mass spectroscopy and was identified as actinonin, a known pseudopeptide antibiotic. The Ki was determined as 1.4 .mu.M and this value was confirmed using pure synthetic actinonin.This publication has 9 references indexed in Scilit:
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