Gluconeogenesis in periportal and perivenous hepatocytes of rat liver, isolated by a new high-yield digitonin/collagenase perfusion technique
- 1 July 1985
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 229 (1), 221-226
- https://doi.org/10.1042/bj2290221
Abstract
A technique is described which allows preparations of hepatocytes, enriched in either periportal or perivenous hepatocytes (‘PP-cells’ and ‘PV-cells’ respectively), in a yield of about 30-50% compared with control cell preparations. The liver is first perfused for 40-60s with digitonin (4 mg/ml) to destroy selectively either the periportal or the perivenous part of the microcirculatory unit, and then the remaining hepatocytes are isolated by the ordinary collagenase perfusion technique. In periportal cells the activities of alanine aminotransferase and pyruvate kinase were 29.4 and 18.7 mumol/min per mg of DNA respectively. The rate of gluconeogenesis was 0.402 mumol/min per mg of DNA. In perivenous cells the corresponding values were 9.55, 22.1 and 0.244 mumol/min per mg of DNA respectively. These data support the concept of a zonation of glucose metabolism within the microcirculatory unit of the liver, with the afferent part (periportal zone) having a 2-fold, more active gluconeogenesis than the efferent part (perivenous zone).This publication has 21 references indexed in Scilit:
- Functional Hepatocellular HeterogeneityHepatology, 2007
- Functional hepatocyte heterogeneity in ammonia metabolism: The intercellular glutamine cycleJournal of Hepatology, 1985
- Predominance of glycolysis in pericentral regions of the liver lobuleEuropean Journal of Biochemistry, 1984
- Selective isolation of intact periportal or perivenous hepatocytes by antero‐ or retrograde collagenase gradient perfusionLiver International, 1983
- Distribution of Pyruvate Kinase Type L and M2 in Microdissected Periportal and Perivenous Rat Liver Tissue with Different Dietary StatesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1983
- Induction in Primary Culture of ‘Gluconeogenic’ and ‘Glycolytic’ Hepatocytes Resembling Periportal and Perivenous CellsEuropean Journal of Biochemistry, 1982
- Liver Cell Heterogeneity. The Distribution of Pyruvate Kinase and Phosphoenolpyruvate Carboxykinase (GTP) in the Liver Lobule of Fed and Starved RatsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976
- Functional heterogeneity of rat liver parenchyma and of isolated hepatocytesFEBS Letters, 1975
- CHANGES IN DISTRIBUTION OF ENZYMES WITHIN THE LIVER LOBULE DURING ADAPTIVE INCREASESJournal of Histochemistry & Cytochemistry, 1972
- CORRELATED MORPHOMETRIC AND BIOCHEMICAL STUDIES ON THE LIVER CELLThe Journal of cell biology, 1969