Methods for the purification of tomato bushy stunt and tobacco mosaic viruses

Abstract

These viruses were isolated and purified employing methods and equipment commonly available. The methods included neutral extractions, and precipitations with (NH4)2SO4 or acid at pH 3.3-5, eliminating previously used procedures considered detrimental to recovery of fully infective virus. The bushy stunt virus was purified until prepns. possessed > 1.4% P and longdash [image]6% carbohydrate. Highly infective prepns. of this virus apparently possessed the same appearance, crystallizability, serol. activity, sedimentation constant (Sw20[degree] = 130 X 10-13) and gross analytical composition as much less infective prepns. previously obtained. Tobacco mosaic virus aggregated in the course of the purification.