Regulation of insulin secretion by cAMP in rat islets of Langerhans permeabilised by high‐voltage discharge
- 15 September 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 205 (2), 205-209
- https://doi.org/10.1016/0014-5793(86)80898-5
Abstract
Adenosine 3',5-cyclic monophosphate (cAMP) was shown to stimulate insulin secretion from electrically permeabilised islets of Langerhans incubated in Ca2+/EGTA buffers. cAMP-induced insulin secretion occurred in the presence of either sub-stimulatory (50 nM) or stimulatory (> 100 nM) concentrations of Ca2+. Similar effects on secretion were obtained in response to 8-bromo-cAMP (8-Br-cAMP) or the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine. Forskolin (0.2–20μM) increased adenylate cyclase activity and enhanced insulin secretion from the permeabilised islets. These results suggest that, in electrically permeabilised islets, cAMP-induced insulin secretion is not dependent on changes in cytosolic Ca2+.Keywords
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