Metabolism of bladder carcinogens. The metabolic path of 2-[8-14C]naphthylamine in the rat

Abstract

Rats injected intraperitoneally with 2-[8-Cl4]-naphthylamine eliminate no radioactivity in the expired CO2- Rats injected intraperitoneally with 2-[8-Cl4] -naphthylamine (approximately 1 mg) retain radioactivity in the blood for periods up to about 9 or 10 weeks. The activity in the blood after 10-15 days is attached almost entirely to the blood cells. Excretion of the radioactive compound or its metabolites is readily demonstrated into the urine and through the bile into the gut. Re-absorption of the amine or its metabolites from the gut after intraperitoneal injection was demonstrated. Excretion of 90-95% of the radioactivity of a single dose in the urine and feces occurs in about 3 days after intraperitoneal injection, but thereafter excretion is very slow so that activity is clearly demonstrable in various organs up till at least 10 weeks after injection.